996-12 Overexpression of Sarcoplasmic Reticulum Calcium ATPase in Neonatal Cardiomyocytes Using a Recombinant Adenovirus

Prevention of Atrial Fibrillation by Using Sarcoplasmic Reticulum Calcium ATPase Pump Overexpression in a Rabbit Model of Rapid Atrial Pacing

BACKGROUND Recent research suggests that abnormal Ca2+ handling plays a role in the occurrence and maintenance of atrial fibrillation (AF). Therefore, Ca2+ release and ingestion depend on properties of the ryanodine receptor (RyR) and sarcoplasmic reticulum Ca2+ATPase2a (SERCA2a). This study aimed to detect whether SERCA2a gene overexpression has a preventive effect on atrial fibrillation cause...

Calcium and lanthanide binding in the sarcoplasmic reticulum ATPase.

The interactions of calcium and lathanides with the sarcoplasmic reticulum ATPase, and their respective ability to activate the enzyme, were studied by direct measurements of binding with radioactive tracers, functional effects on the ATPase partial reactions, changes in the quantum yield of tryptophanyl residues and a covalently bound fluorescein label (fluorescein 5-isothiocyanate, FITC), and...

Cooperative calcium binding and ATPase activation in sarcoplasmic reticulum vesicles.

High affinity calcium binding which is involved in ATPase activation was measured following equilibration of sarcoplasmic reticulum vesicles with Ca2+ buffers in chromatography columns. Binding occurred with ir,,, = 2.3 X lo6 M” and with a maximal level of 8 to 10 m o l per mg of protein. This value corresponds to 2 calcium ions per ATPase phosphorylation site, since the maximal level of phosph...

Ion pumping by calcium ATPase of sarcoplasmic reticulum.

Calcium transport ATPase of canine cardiac sarcoplasmic reticulum. A comparison with that of rabbit fast skeletal muscle sarcoplasmic reticulum.

To define the mechanism responsible for the slow rate of calcium transport by cardiac sarcoplasmic reticulum, the kinetic properties of the Ca2+-dependent ATPase of canine cardiac microsomes were characterized and compared with those of a comparable preparation from rabbit fast skeletal muscle. A phosphoprotein intermediate (E approximately P), which has the stability characteristics of an acyl...